Two rearranged immunoglobulin kappa light chain genes in one mouse myeloma
نویسندگان
چکیده
منابع مشابه
Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.
The organization of the kappa chain constant region gene was compared in DNA from an immunoglobulin-producing mouse myeloma (MOPC 173) and from liver. In situ hybridization using the Southern blotting technique revealed constant region gene-containing EcoRI-DNA fragments of 14 and 20 kb in the myeloma tissue whereas one EcoRI-DNA fragment with a length of 15 kb was found in liver DNA. After enr...
متن کاملFunctional Immunoglobulin Light Chain Genes
Rearrangement of Ig genes proceeds during B cell differentiation in an orderly fashion. First the heavy (H) chain gene is rearranged in pre-B cells . These cells can express cytoplasmic A chain but no light (L) chain (1). The K light chain genes rear range next and if they fail to produce a functional gene on either chromosome the X light chain genes are rearranged (2) . It is generally believe...
متن کاملEvolution of immunoglobulin kappa chain variable region genes in vertebrates.
The major source of immunoglobulin diversity is variation in DNA sequence among multiple copies of variable region (V) genes of the heavy- and light-chain multigene families. In order to clarify the evolutionary pattern of the multigene family of immunoglobulin light kappa chain V region (V kappa) genes, phylogenetic analyses of V kappa genes from humans and other vertebrate species were conduc...
متن کاملEstimation of the number of genes coding for the constant part of the mouse immunoglobulin kappa light chain.
As previously shown, purified 14S RNA of mouse myeloma MOPC-41 forms a single peak on sucrose gradients and gel electrophoresis and codes for a single polypeptide chain, the immunoglobulin light chain produced by the same myeloma in vivo. This 14S mRNA was used for the enzymatic synthesis of DNA (cDNA) which is complementary to the RNA template. A DNA fraction was isolated which has an average ...
متن کاملChromosomal location of human kappa and lambda immunoglobulin light chain constant region genes
The chromosomal location of human constant region light chain immunoglobulin (Ig) genes has been determined by analyzing a group of human fibroblast/rodent somatic cell hybrids with nucleic acid probes prepared from cloned human kappa and lambda constant region genes. Human chromosomes in each cell line were identified by isoenzyme analysis. The DNA from hybrid cells was digested with restricti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1980
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/8.8.1693